News

Job opportunity: Project Manager/Researcher

We are looking for an experienced Project Manager/Researcher to join our team for the EC-funded program ‘VACCELERATE

  • We offer an 17-month contract in a full-time position with a competitive salary 
  • The contract can be further extended depending on performance evaluation and available financing.

Responsibilities

  • Conducts planning for current initiatives and the future state of the project, ensuring and advancing efficient workflows of VACCELERATE project priorities.
  • Leads the coordination and teamwork of research initiatives and ongoing studies of VACCELERATE.
  • Manages the program's communications, outreach and external relations efforts for expanding constituencies within VACCELERATE and outside, including mailings, publications dissemination, website development and press releases; writes a wide variety of material used to promote the program. This includes writing annual reports, executive summaries, strategic plans, by-laws and other strategic reports as necessary.
  • Oversees monthly budget records and coordinates financial and legal responsibilities.
  • Maintains a thorough understanding of COVID-19 vaccine trial development, public health issues around vaccines, and emerging stakeholders in Greece and abroad.
  • Develops new and promotes existing relationships with CLEO contacts, organizations, and institutions in Greece and internationally. This includes liaising with other NGOs, universities and government agencies.
  • Moderates and presents at VACCELERATE meetings, and represents CLEO at meetings and public events, as needed.
  • Supports CLEO’s mission and values and actively looks for ways to improve the organization, e.g. by supporting opportunities and applications to new grants. 

Essential criteria

  • A PhD or Master’s degree in Public health, Infectious Diseases, Vaccine Development or similar relevant topic
  • A minimum of 2 years proven experience working & managing international programs (especially EC-funded programs)
  • Write and speak English fluently 
  • Have excellent IT literacy skills (including experience with data analysis tools)

Additional requirements

  • Strong time management 
  • Enjoys taking initiative
  • Team player
  • Goal-oriented

Interested candidates can apply for this job by sending their CV, a list of scientific publications & a Cover letter to info@cleoresearch.org until the 21st of August  2022.

Greek Infection Prevention Program, GRIPP-SNF, wins 'Gold' at the Patient Partnerships Awards 2022

The Center for Clinical Epidemiology and Outcomes Research - CLEO (https://www.cleoresearch.org) was given the Gold award in the category for Patient Safety for the National Program for the Prevention and Control of Hospital Acquired Infections and Antimicrobial Resistance, "Greek Infection Prevention Program, GRIPP-SNF", at the Patient Partnerships Awards 2022 (https://www.patientawards.gr/) organized by Health Daily of BOUSSIAS Communications.

The award comes as a reward for the many months of efforts of all organizations involved in GRIPP-SNF, namely the National Agency for Quality Assurance in Health S.A. (AQAHS.A.), the National and Kapodistrian University of Athens (NKUA), the Greek Ministry of Health (MoH), the National Public Health Organization (NPHO), and the Institute for Healthcare Improvement (IHI), together with the Center for Clinical Epidemiology and Outcomes Research (CLEO), highlighting the importance of collaboration when it comes to patient safety and protecting patients from hospital-acquired infections.

Congratulations are due to all members of the CLEO team who actively participate in the planning and implementation of the "GRIPP-SNF" program, which is implemented in 10 major hospitals of the country. Also, special mention goes to the Infection Prevention Nurses at the hospitals participating in the program, who every day make efforts to protect their patients and improve the quality of the health services provided.

Finally, a big "thank you" goes to the Stavros Niarchos Foundation (SNF), which is exclusively funding this project, within the SNF "Health Initiative" Program (https://www.snfhi.org/).

Gut Microbiome: The mirror of our health

Written by Dimitra Kousi, Junior Biostatistician, CLEO.

What is the microbiome?

Trillions of microorganisms colonize our gastrointestinal tract, skin, genito-urinary system etc., together forming the ‘human microbiome’. Most of these microorganisms are bacteria, but the microbiome also includes fungi, protozoa and viruses. Some of these microorganisms have a beneficial effect on our health, others have been associated with harmful functions while others remain neutral. 

Recent studies on the human microbiome showed that 56% of the total cell count in our body are not human cells, but bacterial, weighing nearly 0,2 kg (almost as much as a zucchini!) (1). The microbiome is unique for each and every one of us and, in a way, constitutes our identity (2). The vast majority of the human microbial communities inhabit the gastrointestinal tract, and this is the reason why the gut microbiome (GM) has attracted the most attention.

How can we study the gut microbiome?

Rapid advancements in the fields of metagenomics and bioinformatics in the last few decades have shed light on the world of the GM (3). Following the collection of a fecal sample from a person, the genetic material of microorganisms can be isolated. Gene sequencing techniques like 16S rRNA and Shotgun Metagenomic Sequencing are the most common methods used to analyze the genetic material of the sample and describe the GM’s composition, as well as its interactions with the host. It is worth mentioning that Next Generation Sequencing technologies have significantly contributed to the improvement of the efficiency of the aforementioned techniques while also reducing their cost. Together with the advent of Proteomics (4) and Metabolomics (5) that study the protein activity and metabolic processes in our body, respectively, we can get closer to “unlocking” the GM mystery.

What is the role of the gut microbiome in improving our health?

A healthy GM is generally considered to be one with high levels of diversity and which preserves a stable and balanced composition (6, 7). Maintaining a healthy GM has been shown to play an important role in:

  • Regulating our metabolism, by contributing to the fermentation of non-digestible food components and harvesting of nutrients
  • Synthesizing important vitamins, amino acids, as well as metabolic by-products (e.g., butyric acid, propionic acid) that support the functions of the large-intestine
  • Boosting our immune system
  • Fighting off infections caused by pathogens
  • Modulating brain function

Which factors influence the gut microbiome?

Some of the factors that can affect the gut microbiome of a human host are (8, 9):

  • Environment
  • Dietary habits
  • Lifestyle (exercise, smoking, anxiety, etc.)
  • Drug and antibiotic use
  • Childbirth delivery method (natural vaginal delivery, cesarean section)
  • Diseases – Chronic conditions
  • Hormonal changes
  • Aging

How does our gut microbiome change over the years?
From the moment of birth and up until the ages of 3 to 5, the GM changes drastically and it is mainly affected by factors such as environment, diet and antibiotic use (10). Following these crucial first years, the GM acquires an adult-like form that remains relatively stable in later years (11). After the age of 65, the GM’s diversity decreases, while disease-associated microorganisms become more prevalent (12, 13).

Gut microbiome and disease

The disruption of the GM’s balance, also known as ‘dysbiosis’, has been associated with numerous diseases and conditions (14, 15). However, the exact mechanisms of this relationship have not been completely elucidated and remain to be further investigated. Examples of diseases that appear to be linked to ‘dysbiosis’ are:

  • Inflammatory bowel disease
  • Obesity
  • Cardiovascular disease
  • Diabetes
  • Autoimmune diseases, such as psoriasis, autism, etc
  • Depression
  • Neurological disorders, such as Parkinson’s disease, Alzheimer’s disease, etc.
  • Cancer
  • Allergies

What can we do?

Even though we cannot choose our birth delivery method or freeze time, so that we never get old, there are several GM-altering factors that we can control to some extent. Balanced diet, regular exercise, proper use of antibiotics, stress relieving techniques, are a few examples of what we can do to preserve a healthy GM.

With regards to diet, the use of prebiotics and probiotics has been proven to play a significant role in attaining metabolic homeostasis and strengthening the immune system.

Prebiotics, which are undigested carbohydrates that end up in the lowest part of the gastrointestinal tract, are “food” for the beneficial bacteria of the GM, therefore promoting their growth (16). The best-known sources of prebiotics are fruits and vegetables, but there are also prebiotic supplements.

On the other hand, probiotics are precisely those beneficial microorganisms of the GM (17). We can strengthen their presence by consuming certain foods, such as yogurt, peas, sauerkraut, etc., or through food supplements. Regulated intake of these microorganisms may contribute to the maintenance or restoration of the GM’s balance.

Innovative Microbiome-Based Therapies

  • Fecal Microbiota Transplantation (FMT) from healthy donors to people who have been infected by Clostridium difficile is a novel therapeutic method that has been proven to be remarkably efficient (18). There is hope that stool transplant will turn out to be equally successful in treating conditions, such as obesity, several gastrointestinal disorders, etc.
  • Psychobiotics, probiotics that convey mental health benefits to the host (19, 20), could emerge as a promising alternative to the existing psychotropic medications aiming to fight depression, anxiety and behavioural disorders. Many questions that concern the gut-brain connection and the underlying mechanisms of their communication remain currently unanswered. However, what we can say with certainty is that once we manage to lift that veil, the field of Psychiatry will be irrevocably changed.

 

Read more about CLEO's relevant research programme examining the microbiome in the "Blue Microbiome study", here

 

References

  1. Sender, R., Fuchs, S. & Milo, R. Revised Estimates for the Number of Human and Bacteria Cells in the Body. PLoS Biol 14, e1002533 (2016).
  2. Gilbert, J. A. et al. Current understanding of the human microbiome. Nat Med 24, 392–400 (2018).
  3. Bharti, R. & Grimm, D. G. Current challenges and best-practice protocols for microbiome analysis. Briefings in Bioinformatics 22, 178–193 (2021).
  4. Aslam, B., Basit, M., Nisar, M. A., Khurshid, M. & Rasool, M. H. Proteomics: Technologies and Their Applications. J Chromatogr Sci 55, 182–196 (2017).
  5. Belizário, J. E. & Faintuch, J. Microbiome and Gut Dysbiosis. in Metabolic Interaction in Infection (eds. Silvestre, R. & Torrado, E.) vol. 109 459–476 (Springer International Publishing, 2018).
  6. Rinninella, E. et al. What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases. Microorganisms 7, 14 (2019).
  7. Valdes, A. M., Walter, J., Segal, E. & Spector, T. D. Role of the gut microbiota in nutrition and health. BMJ k2179 (2018) doi:10.1136/bmj.k2179.
  8. Hasan, N. & Yang, H. Factors affecting the composition of the gut microbiota, and its modulation. PeerJ 7, e7502 (2019).
  9. Candela, M. et al. Dynamic efficiency of the human intestinal microbiota. Critical Reviews in Microbiology 41, 165–171 (2015).
  10. Rodríguez, J. M. et al. The composition of the gut microbiota throughout life, with an emphasis on early life. Microbial Ecology in Health & Disease 26, (2015).
  11. Vemuri, R. et al. Gut Microbial Changes, Interactions, and Their Implications on Human Lifecycle: An Ageing Perspective. BioMed Research International 2018, 1–13 (2018).
  12. Salazar, N., Valdés-Varela, L., González, S., Gueimonde, M. & de los Reyes-Gavilán, C. G. Nutrition and the gut microbiome in the elderly. Gut Microbes 8, 82–97 (2017).
  13. Ragonnaud, E. & Biragyn, A. Gut microbiota as the key controllers of “healthy” aging of elderly people. Immun Ageing 18, 2 (2021).
  14. Durack, J. & Lynch, S. V. The gut microbiome: Relationships with disease and opportunities for therapy. Journal of Experimental Medicine 216, 20–40 (2019).
  15. Wang, B., Yao, M., Lv, L., Ling, Z. & Li, L. The Human Microbiota in Health and Disease. Engineering 3, 71–82 (2017).
  16. Wang, S. et al. Rational use of prebiotics for gut microbiota alterations: Specific bacterial phylotypes and related mechanisms. Journal of Functional Foods 66, 103838 (2020).
  17. Kim, S.-K. et al. Role of Probiotics in Human Gut Microbiome-Associated Diseases. Journal of Microbiology and Biotechnology 29, 1335–1340 (2019).
  18. Gupta, S., Allen-Vercoe, E. & Petrof, E. O. Fecal microbiota transplantation: in perspective. Therap Adv Gastroenterol 9, 229–239 (2016).
  19. Del Toro-Barbosa, M., Hurtado-Romero, A., Garcia-Amezquita, L. E. & García-Cayuela, T. Psychobiotics: Mechanisms of Action, Evaluation Methods and Effectiveness in Applications with Food Products. Nutrients 12, 3896 (2020).
  20. Sarkar, A. et al. Psychobiotics and the Manipulation of Bacteria–Gut–Brain Signals. Trends in Neurosciences 39, 763–781 (2016).

 

 

CLEO COVID-19 KAP Community Study – Press Release

The COVID-19 pandemic has presented unique, unprecedented challenges for national healthcare systems. The faltering global response - characterized by a lack of preparedness among health care institutions, workers and the wider community - has underscored the importance

of investing in public health research. However, it has also provided a unique opportunity for countries to diagnose and treat gaps within their own capacity for disease prevention. 

Like other countries, Greece has turned to urgent measures such as social distancing, quarantining, travel restrictions and border closures to rapidly halt transmission, reduce the risk of illness and protect healthcare systems from overfilling. Public misconceptions about transmissible diseases play a large part in adherence to these measures, and in the case of COVID, may have prevented the most effective response to the pandemic.

With this knowledge in mind, the proposed study aimed to explore knowledge and perceptions held across the Greek community about COVID-19, and whether these attitudes might be tied to changes in behaviors regarding other prevention practices (e.g. vaccination). The results of the study could be used to guide qualitative studies such as selected focus groups within the community, to more deeply examine the underlying motives for behavior change in relation to the uptake of preventative health practices. Ultimately, the goal of this exploration is to improve both individual and collective preparedness for future epidemic waves.

The study

This telephone survey study is one of the first studies examining the knowledge, attitudes, and practices (KAP) regarding COVID-19 in the Greek general population, as well as the first to identify core areas of concern for optimal community intervention. Participants in the study were surveyed by telephone on their KAP related to COVID-19. Participants ranged from 18 to 90 years old, and a third of respondents reported that they belonged to a vulnerable group.

Knowledge

Participants were asked to respond to COVID-19 knowledge items as either yes or no. Higher scores indicated better knowledge of COVID-19. The majority of participants named that they use media as their primary source of information about the pandemic, which suggests media as a channel to influence KAP and spark behavior change. According to analysis, women and those above 24 were more likely to know the 5 most common COVID-19 related symptoms. Handwashing and safe distancing were the most popular prevention methods among those surveyed (Fig. 1).

Figure 1. Which of the following are preventative measures against the spread of coronavirus

Figure 1. “Which of the following are preventative measures against the spread of coronavirus?”

As for knowledge about how COVID-19 is transmitted, 11.2% of respondents thought that coronavirus could be transmitted through animals or mosquitoes, and 27.6% through food (Fig. 2).

Figure 2. In which of the following ways is the coronavirus transmitted

Figure 2. “In which of the following ways is the coronavirus transmitted?”

Αξίζει να σημειωθεί ότι η πλειοψηφία των συμμετεχόντων στην έρευνα ανέφερε πως τα ΜΜΕ είναι η κύρια πηγή πληροφόρησης και ενημέρωσης για την πανδημία, γεγονός το οποίο επιβεβαιώνει ότι αυτά αποτελούν βασικό δίαυλο που επηρεάζει την κοινή γνώμη και θα μπορούσαν να προκαλέσουν αλλαγή συμπεριφοράς.

 

Attitudes

Participants were asked to respond to COVID-19 knowledge items as either yes or no. Higher scores indicated better knowledge of COVID-19. The majority of participants named that they 

The study also looked at the general public’s attitudes and perceptions about coronavirus. The same group, women and those above 24, was more likely to believe that coronavirus was developed by humans in laboratories (Fig. 3).

Figure 3. Do you believe that the novel coronavirus was made by human in the laboratory

Figure 3. “Do you believe that the novel coronavirus was made by human in the laboratory?”

 Practices

Members of a vulnerable group noted that the coronavirus pandemic improved their attitude towards vaccines. Furthermore, those who marked that coronavirus improved their attitude about vaccines named that they were more likely to receive the upcoming flu vaccine and the COVID-19 vaccine, when it becomes available.

Figure 4. Attitudes towards vaccination

Figure 4. Attitudes towards vaccination

Similarly, those who believed the transmission of coronavirus is “much more” likely than H1N1 were significantly more likely to wash their hands more and state that they would get the upcoming COVID-19 vaccine when available (Fig. 5). Those who beleived that SARS-CoV2 is deadlier (Fig. 6) were similarly more positive towards a COVID-19 vaccine.

 Figure 5. How contagious do you believe the novel coronavirus is compared to the H1N2 flu

Figure 5. “How contagious do you believe the novel coronavirus is compared to the H1N2 flu?”

 Figure 6. How deadly do you believe the novel coronavirus is compared to the H1N2 flu

Figure 6. “How deadly do you believe the novel coronavirus is compared to the H1N2 flu?”

Finally, participants were asked about their motivation to enter social isolation. Both the fear of transmitting it to others and the desire to reduce the spread of infection within one’s community were the most popular, while the fear for one’s own life was the least popular among respondents. This highlights an important avenue for improving public adherence to social distancing and quarantine guidelines by emphasizing a respect for the common good.

Overall, this study showed that the general public’s knowledge, attitudes, and practices are influenced by identity markers. It also showed that specific perceptions about transmissible diseases can improve public adherence to control measures, which play a decisive role in reducing infection and controlling the spread of disease. Given the fact that there is neither an effective cure nor a vaccine available for COVID-19, and that there is a second pandemic wave expected, these understandings are extremely valuable in preparing the Greek healthcare system and general population to deal with future public health crises. The COVID-19 pandemic has presented unique, unprecedented challenges for national healthcare systems. The faltering global response - characterized by a lack of preparedness among health care institutions, workers and the wider community - has underscored the importance of investing in public health research. However, it has also provided a unique opportunity for countries to diagnose and treat gaps within their own capacity for disease prevention.